Articles | Volume 4, issue 2
Primate Biol., 4, 153–162, 2017

Special issue: Stem cells in non-human primates

Primate Biol., 4, 153–162, 2017

Research article 18 Aug 2017

Research article | 18 Aug 2017

Overcoming barriers to reprogramming and differentiation in nonhuman primate induced pluripotent stem cells

Jacob J. Hemmi et al.

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Cited articles

Chen, G., Gulbranson, D. R., Hou, Z., Bolin, J. M., Ruotti, V., Probasco, M. D., Smuga-Otto, K., Howden, S. E., Diol, N. R., Propson, N. E., and Wagner, R.: Chemically defined conditions for human iPSC derivation and culture, Nat. Methods, 8, 424–429,, 2011.
Chetty, S., Pagliuca, F. W., Honore, C., Kweudjeu, A., Rezania, A., and Melton, D. A.: A simple tool to improve pluripotent stem cell differentiation, Nat. Methods, 10, 553–556, pmid:23584186, 2013.
Choi, J., Lee, S., Clement, K., Mallard, W., Tagliazucchi, G.M., Lim, H., Choi, I. Y., Ferrari, F., Tsankov, A., Pop, R., Lee, G., Rinn, J., Meissner, A., Park, P. J., and Hochedlinger, K.: A comparison of genetically matched cell lines reveals the equivalence of human iPSCs and ESCs, Nat. Biotechnol., 33, 1173–1181,, 2016.
Choi, S. C., Choi, J. H., Cui, L. H., Seo, H. R., Kim, J. H., Park, C. Y., Joo, H. J., Park, J. H., Hong, S. J., Yu, C. W., and Lim, D. S.: Mixl1 and Flk1 and key players of Wnt/TGF-β signalling during DMSO-induced differentiation in P19 cells, J. Cell. Physiol., 230, 1807–1821,, 2015.
Farnsworth, S. L., Qiu, Z., Mishra, A., and Hornsby, P. J.: Directed neural differentiation of induced pluripotent stem cells from non-human primates, Exp. Biol. M., 238, 276–284,, 2013.
Short summary
In these studies induced pluripotent stem cells (iPS cells) were generated from donors across the life span of the common marmoset (Callithrix jacchus) and were subjected to a directed neural differentiation protocol. Additionally chimpanzee (Pan troglodytes) iPS cells were generated. Both aged marmoset iPS cells and chimpanzee iPS cells showed defects in their ability to differentiate. However, most of these defects were able to be overcome by a brief treatment with dimethyl sulfoxide.